Microbiological and serological control of Escherichia coli O157: H7 in kindergarten staff in Buenos Aires city and suburban areas.
Fernandez-Brando, R. J., Amaral, M. M., Ciocchini, A. E., Bentancor, L. V., Trelles, J. A., Da Rocha, M., Landriel, M., Ugarte, M., Briones, G., Ibarra, C. and Palermo, M. S.
Laboratorio de Patogenesis y de Procesos Infecciosos, Instituto de Medicina Experimental, (IMEX-CONICET), Academia Nacional de Medicina, Argentina.
Laboratorio de Fisiopatogenia, Departamento de Fisiologia, Instituto de Fisiologia y Biofisica Bernardo Houssay (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Instituto de Investigaciones Biotecnologicas Rodolfo Ugalde-Instituto Tecnologico de Chascomus (IIB-INTECH), Universidad Nacional de San Martin (UNSAM), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina.
Laboratorio de Ingenieria Genetica y Biologia Celular y Molecular, Universidad Nacional de Quilmes, Buenos Aires, Argentina.
Laboratorio de Investigaciones en Biotecnologia Sustentable (LIBioS), Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina.
Asociacion Lucha contra el Sindrome Uremico Hemolitico (LuSUH), Argentina.
Catedra de Nutricion, Carrera de Tecnologia de Alimentos, Universidad Nacional de Lanus, Buenos Aires, Argentina.
Laboratorio de Patogenesis y de Procesos Infecciosos, Instituto de Medicina Experimental, (IMEX-CONICET), Academia Nacional de Medicina, Argentina. E-mail: marinaspalermo@hotmail.com.
Shiga toxin (Stx)-producing Escherichia coli (STEC) infections are implicated in the development of the life-threatening hemolytic-uremic syndrome (HUS). Despite the magnitude of the social and economic problems caused by HUS, no licensed vaccine or effective therapy is currently available for human use. Prevention of STEC infections continues being the most important measure to reduce HUS incidence. This is especially true for Argentina where HUS incidence among children is extremely high and shows an endemic pattern. The aim of this work was to investigate serologically adult staff of kindergartens in Buenos Aires city and suburban areas in order to detect possible carriers, and to educate personnel about good practices to reduce HUS transmission. We also assessed the microbiological quality of water and meal samples from the same kindergartens. We tested 67 healthy adults, 13 water supplies and 6 meals belonging to 6 public kindergartens. We analysed hand swabs for isolation of STEC and serum samples for the presence of antibodies against Stx and lipopolysaccharide (LPS) of O157 serogroup. We identified 46 Stx2-positive individuals, but only 7 for O157 LPS. No presence of STEC pathogens was detected in hands of staff, water or meal samples.
Medicina (Buenos Aires) 77(3): 185-190 (2017)