Sex hormone binding globulin: Expression throughout early development and adult Pejerrey fish, Odontesthes bonariensis.
González, A., Fernandino, J.I., Hammond, G.L and Somoza, G.M.
Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico de Chascomus (IIB-INTECH). CONICET-UNSAM. Chascomus, Buenos Aires, Argentina.
Department of Cellular and Physiological Sciences. Faculty of Medicine. The University of British Columbia, British Columbia, Canada.
Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico de Chascomus (IIB-INTECH). CONICET-UNSAM. Chascomus, Buenos Aires, Argentina. Electronic address: somoza@intech.gov.ar.
Sex hormone binding globulin (Shbg) is a plasma glycoprotein that binds and transports steroids in the blood of all vertebrate classes apart from birds. In the present study we characterized shbg from pejerrey, a fish species with a well characterized temperature-dependent sex determination. The pejerrey shbg mRNA comprises 1185 bp encoding for a 395 amino acid Shbg precursor protein that includes a leader sequence for secretion. Relative quantification of shbg transcript abundance revealed expression early in development coinciding with the sex-determining period and probably in association with temperature leading to male determination. The hepatopancreas was the main site of shbg expression, which varied according to the sex cycle in females. It was also expressed in gills, gonads, gut and taste buds during both larval stages and in adult fish. The presence of Shbg in organs in close contact with the environment such as gills, pseudobranchs, gut and taste buds suggests that these are potential sources of uptake or release of steroids/xenosteroids to and from the aquatic environment.
General and Comparative Endocrinology 247: 205-214 (2017)